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First investigation of spider silk as a braided microsurgical suture

✍ Scribed by Joern W. Kuhbier; Kerstin Reimers; Cornelia Kasper; Christina Allmeling; Anja Hillmer; Björn Menger; Peter M. Vogt; Christine Radtke


Book ID
102300232
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
418 KB
Volume
97B
Category
Article
ISSN
1552-4973

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✦ Synopsis


Abstract

Inhibition of axonal outgrowth accompanied by neuroma formation appears in microsurgical nerve repair as reaction to common microsuture materials like silk, nylon, or polyglycolic acid. In contrast, recent findings revealed advantages of spider silk fibers in guiding Schwann cells in nerve regeneration. Here, we asked if we could braid microsutures from native spider silk fibers. Microsutures braided of native spider dragline silk were manufactured, containing either 2 × 15 or 3 × 10 single fibres strands. Morphologic appearance was studied and tensile strength and stress‐strain ratio (SSR) were calculated. The constructed spider silk sutures showed a median thickness of 25 μm, matching the USP definition of 10‐0. Maximum load and tensile strength for both spider silk microsutures were significantly more than 2‐fold higher than for nylon suture; SSR was 1.5‐fold higher. All values except elasticity were higher in 3 × 10 strand sutures compared to 2 × 15 strand sutures, but not significantly. In this pilot study, we demonstrate the successful manufacture of microsutures from spider silk. With regards to the mechanical properties, these sutures were superior to nylon sutures. As spider silk displays high biocompatibility in nerve regeneration, its usage in microsurgical nerve repair should be considered. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.