Fine structural changes in the mandibular gland of the male spider crab, Libinia emarginata (L). Following eyestalk ablation

✍ Scribed by Gertrude W. Hinsch

• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 874 KB
• Volume: 154
• Category: Article
• ISSN: 0362-2525
• DOI: 10.1002/jmor.1051540208

✦ Synopsis

Abstract

Mandibular glands from destalked male spider crabs were fixed in glutaraldehyde‐paraformaldehyde, post‐fixed in 1% osmium in phosphate buffer, dehydrated in acetone and embedded in Spurr's low‐viscosity medium ('69). The glands hypertrophy and several changes occur in the subcellular cytoarchitecture following eyestalk ablation. These include loss of most of the smooth endoplasmic reticulum or its appearance as lamallae, appearance of numerous polysomes and a change in mitochondrial structure. Large vesicles with ribosomes spaced at intervals along their membranes are apparently formed by blebbing of the outer nuclear membrane and appear throughout the cytoplasm. Banded structures consisting of alternate granular and flocculent materials are found within the nucleus.