Fine-needle aspiration cytology combined with flow cytometry immunophenotyping is a rapid and accurate approach for the evaluation of suspicious superficial lymphoid lesions
✍ Scribed by Claire Mathiot; Didier Decaudin; Jerzy Klijanienko; Jérôme Couturier; Anne Salomon; Janine Dumont; Philippe Vielh
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 105 KB
- Volume
- 34
- Category
- Article
- ISSN
- 8755-1039
- DOI
- 10.1002/dc.20487
No coin nor oath required. For personal study only.
✦ Synopsis
The authors report a prospective study on 88 samples of superficial lesions (lymph nodes, skin nodules, and breast tumors), performed by fine-needle aspiration cytology (FNAC) in 74 patients, allowing (i) morphologic analysis combined with immunophenotyping by flow cytometry (FCM) and (ii) a cytogenetic study in 33 cases.
Thirty-nine FNAC (44.3%) were performed at the time of diagnosis. The cytology results were correlated with histopathologic examination in 32 cases. Forty-nine FNAC (55.7%) were performed in the context of follow-up of a lymphoma and the results were correlated with those of histopathologic examination in 14 cases.
In this study, the concordance between FNAC plus FCM and histopathologic examination was 90% for low-grade non-Hodgkin's malignant lymphomas (NHLs) and 83% for high-grade NHL.
The limits of this morphologic and phenotypic approach are (i) partial tumor infiltrations, (ii) Hodgkin lymphoma, and (iii) T-cell NHL.
In conclusion, it may be said that this combined approach is very useful for diagnosis and follow-up of patients but requires teams experienced in the sampling technique and the morphologic diagnosis of the various types of low-grade NHL in which supplementary ancillary studies may be performed when mor-phology and flow cytometry immunophenoyping are not conclusive.