Fine mapping of human PI 3-kinase associated p85α transcripts in the YAC contig surrounding the spinal muscular atrophy gene
✍ Scribed by Jana Huschenbett; Alexander Gasch; Andrea Katzer; Astrid Speer
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 466 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0340-6717
No coin nor oath required. For personal study only.
✦ Synopsis
During a search for transcribed sequences within the gene region for autosomal recessive spinal muscular atrophy (SMA), two cDNA clones were isolated from a human fetal brain and an adult spinal cord cDNA library, respectively, by use of the cosmid LA96B (LAS96). The clones sized 950 bp and 1733 bp detect a 7.7-kb transcript in all tested human tissues. An additional transcript of 6.6 kb is detectable in brain and kidney, and faintly in skeletal muscle and liver. Using comparative human Northern blot analysis, the isolated LA96B cDNA clones could be identified as parts of the 3" untranslated region from the phosphatidylinositol 3 (PI 3)-kinase associated p85c~ transcripts; these were unknown up to now. The 5" end of the gene was mapped to YAC-EFTA:A, whereas the 3" end was localized within the distal overlapping YAC 85 flanking the SMA candidate gene region.