Fine mapping and functional activity of the adenosine deaminase origin in murine embryonic fibroblasts
✍ Scribed by Sahar Sibani; Emmanouil Rampakakis; Domenic Di Paola; Maria Zannis-Hadjopoulos
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 334 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0730-2312
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
DNA replication initiates at origins within the genome. The late‐firing murine adenosine deaminase (mAdA) origin is located within a 2 kb fragment of DNA, making it difficult to examine by realtime technology. In this study, fine mapping of the mAdA region by measuring the abundance of nascent strand DNA identified two origins, mAdA‐1 and mAdA‐C, located 397 bp apart from each other. Both origins conferred autonomous replication to plasmids transfected in murine embryonic fibroblasts (MEFs), and exhibited similar activities in vivo and in vitro. Furthermore, both were able to recruit the DNA replication initiator proteins Cdc6 and Ku in vitro, similar to other bona fide replication origins. When tested in a murine Ku80^−/−^ cell line, both origins exhibited replication activities comparable to those observed in wildtype cells, as did the hypoxanthine‐guanine phosphoribosyltransferase (HPRT) and c‐myc origins. This contrasts with previously published studies using Ku80‐deficient human cells lines and suggests differences in the mechanism of initiation of DNA replication between the murine and human systems. J. Cell. Biochem. 104: 773–784, 2008. © 2008 Wiley‐Liss, Inc.