## Abstract Frequencyβdomain fluorescence lifetime imaging microscopy (FLIM) has become a commonly used technique to measure lifetimes in biological systems. However, lifetime measurements are strongly dependent on numerous experimental parameters. Here, we describe a complete calibration and chara
β¦ LIBER β¦
Finding of Optimal Calcium Ion Probes for Fluorescence Lifetime Measurement
β Scribed by Keisuke Yoshiki; Hiroki Azuma; Kazuhiko Yoshioka; Mamoru Hashimoto; Tsutomu Araki
- Publisher
- Optical Society of Japan
- Year
- 2005
- Tongue
- English
- Weight
- 246 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1340-6000
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The binding properties of trivalent metal ions to polyelectrolytes were investigated through the use of terbium [Tb(lII)] in fluorescence studies. Tbe fluorescence intensity and lifetimes of the Ianthanide ions are directly dependent upon the number of water molecules bound to their inner coordinati