Filter-binding assay procedure for thyro
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Akira Inoue; Junichi Yamakawa; Munehiko Yukioka; Seiji Morisawa
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Article
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1983
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Elsevier Science
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English
โ 666 KB
An assay procedure for thyroid hormone receptor activity which used nitrocellulose membrane filters was developed. Receptor proteins, extracted from washed rat liver nuclei with a 0.4 M NaCl solution, were incubated with 125I-labeled thyroid hormone (T3), and filtered on the cellulose ester membrane