Filipin as a Fluorescent Probe of Lipoprotein-Derived Sterols on Thin-Layer Chromatograms
✍ Scribed by Gary B. Smejkal; George Hoppe; Henry F. Hoff
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 98 KB
- Volume
- 239
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The main disadvantage of this method is its low sen-lective affinity of filipin for cholesterol and structurally related sterols substantiates its use as a probe in stud-sitivity, which is caused by the fact that the substrate, rather than cleavage product, is detected. Thus, the ies of biological membranes and lipoproteins. Previously, filipin has been used for cholesterol analysis ratio of cleaved to uncleaved product must be relatively higher than that required in gel-based radioactive of mitochondrial membranes (1), viral envelopes (2), and clathrin-coated pits associated with prion endocy-methods. Calibration with radioactive nucleotides would be necessary to obtain quantitative measure-tosis (3). Filipin has also been used to demonstrate decreases in membrane cholesterol coinciding with in-ments of nucleotide released.
While this method is not suitable for kinetic mea-creased membrane fluidity as occurs in the transition from cis to trans Golgi cisternae (4) and in acrosomal surements, benefits should reveal themselves in largescale screening for candidate inhibitors.
membranes during sperm capacitation (5). Clinically, filipin has been used to screen aminiotic fluid and chorionic villus cells for prenatal diagnosis of Niemann-