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Field-amplified on-line sample stacking for determination of carnosine-related peptides by capillary electrophoresis

✍ Scribed by Ying Huang; Yanfang Shi; Jianping Duan; Guonan Chen

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
525 KB
Volume
29
Category
Article
ISSN
1615-9306

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✦ Synopsis

Field-amplified on-line sample stacking for determination of carnosine-related peptides by capillary electrophoresis

An on-line sample stacking method, namely field-amplified sample injection, has been developed for the separation and determination of carnosine, anserine, and homocarnosine by capillary electrophoresis. Using electrokinetic injection, about 130-to 160-fold improvement of sensitivity was achieved without loss of separation efficiency when compared to conventional sample injection. For conventional injection, the samples were dissolved in running buffer and then hydrodynamically injected for 10 s (3.45 kPa). Various parameters affecting separation and sample stacking were optimized. Under optimum conditions, linear responses were obtained over two orders of magnitude and the detection limits (defined as S/N = 3) of carnosine, anserine, and homocarnosine were 1.5610 -8 to 1.6610 -8 mol/L.

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