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Fibronectin stimulates Escherichia coli phagocytosis by microglial cells

✍ Scribed by Sandra Ribes; Sandra Ebert; Tommy Regen; Dirk Czesnik; Jörg Scheffel; Andre Zeug; Stephanie Bunkowski; Helmut Eiffert; Uwe-Karsten Hanisch; Sven Hammerschmidt; Roland Nau

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
537 KB
Volume
58
Category
Article
ISSN
0894-1491

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✦ Synopsis

Abstract

Microglia express Toll‐like receptors (TLRs) that recognize invading pathogens as well as endogenous proteins such as fibronectin under nonphysiological conditions. Here, we demonstrated that fibronectin stimulates murine microglia in culture in a dose‐dependent manner: microglial cells secreted proinflammatory cytokines and chemokines and increased phagocytosis of Escherichia coli DH5α and E. coli K1 strains. Low levels of fibronectin exerted a synergistic effect on the release of proinflammatory compounds by microglia co‐stimulated with agonists for TLR1/2 (Pam~3~CSK~4~) or TLR9 (CpG DNA), but not in combination with the TLR4 agonist lipopolysaccharide (LPS). Phagocytosis of bacterial strains was moderately enhanced when microglia was co‐stimulated with high concentrations of fibronectin and one pathogen‐derived TLR agonist. In conclusion, fibronectin increased proinflammatory and phagocytotic functions in microglia and partially synergized with microbial TLR agonists. © 2009 Wiley‐Liss, Inc.

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