Fibroblast-specific perturbation of transforming growth factor β signaling provides insight into potential pathogenic mechanisms of scleroderma-associated lung fibrosis: Exaggerated response to alveolar epithelial injury in a novel mouse model
✍ Scribed by Rachel K. Hoyles; Korsa Khan; Xu Shiwen; Sarah L. Howat; Gisela E. Lindahl; Patricia Leoni; Roland M. du Bois; Athol U. Wells; Carol M. Black; David J. Abraham; Christopher P. Denton
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 860 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Objective
To explore increased susceptibility to fibrosis following experimental injury to alveolar epithelial cells (AECs) in a novel transgenic mouse model of scleroderma with fibroblast‐specific perturbation of transforming growth factor β (TGFβ) signaling (TβRIIΔk‐fib mice).
Methods
Wild‐type (WT) and transgenic mice were injured with intratracheally administered saline or bleomycin, and the lungs were harvested for biochemical, histologic, and electron microscopic analysis.
Results
Electron microscopy revealed AEC abnormalities in the lungs of untreated transgenic mice and bleomycin‐treated WT mice; the lungs of transgenic mice treated with bleomycin showed severe epithelial damage. Compared with lungs from bleomycin‐treated WT mice, lungs from bleomycin‐treated transgenic mice demonstrated increased fibroproliferation, myofibroblast persistence, and impaired hyperplasia and increased apoptosis of type II AECs. The lungs from saline‐treated transgenic mice and those from bleomycin‐treated WT mice had phenotypic similarities, suggesting enhanced susceptibility to minor epithelial injury in the transgenic strain. The level of collagen was increased in the lungs from transgenic mice compared with that in the lungs from WT mice after treatment with either bleomycin or saline. Persistent fibrosis in bleomycin‐treated transgenic mice was independent of ongoing neutrophil inflammation but was associated with impaired alveolar epithelial repair.
Conclusion
These results suggest that in the context of fibroblast‐specific perturbation of TGFβ signaling, even minor epithelial injury induces significant fibrosis. The model supports a central role for TGFβ in determining fibrosis and demonstrates that lung fibroblasts may regulate the response of AECs to injury. Our findings provide insight into likely pathogenic mechanisms in scleroderma‐associated pulmonary fibrosis.