Fibrinolytic assay based on turbidity of polystyrene particles released from the clot
✍ Scribed by Richard E. Maxwell; Vera Lewandowski; Deena Allen
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 330 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The lysis of fibrin clots can be quantitatively measured by analyzing solubilized clot material, or dyes which have been incorporated into the clot, as these substances are released into fluids contacting the clot (1). These measurements have to be made at certain wavelengths, and if one wishes to study the effects of compounds which themselves absorb at those wavelengths, this can be done by using radioactive labels in the clot (2). However, we have long felt the need for a more simple solution to this problem. Ferreira (3) described a modified "latex" fixation test for serum fibrinolytic activity, but this was not applicable to plasma and we were unable to obtain satisfactory results in purified component systems. Further efforts to take advantage of the measurement of "latex" turbidity resulted in the method to be descrihcd.