𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fibrinogen Patterns and Activity on Substrates with Tailored Hydroxy Density

✍ Scribed by José Carlos Rodríguez Hernández; Patricia Rico; David Moratal; Manuel Monleón Pradas; Manuel Salmerón-Sánchez

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
808 KB
Volume
9
Category
Article
ISSN
1616-5187

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The influence of the surface fraction of OH groups on fibrinogen (FG) adsorption is investigated in copolymers of ethyl acrylate and hydroxy ethylacrylate. The amount of adsorbed FG, quantified by western‐blotting combined with image analysis of the corresponding bands, decreases as the hydrophilicity of the substrate increases. The influence of substrate wettability on FG conformation and distribution is observed by atomic force microscopy (AFM). The most hydrophobic substrate promotes FG fibrillogenesis, which leads to a fibrin‐like appearance in the absence of any thrombin. The degree of FG interconnection was quantified by calculating the fractal dimension of the adsorbed protein from image analysis of the AFM results. The biological activity of the adsorbed FG is correlated to cell adhesion on FG‐coated substrates.

magnified image

📜 SIMILAR VOLUMES

Fibronectin activity on substrates with
Fibronectin activity on substrates with controlled OH density
✍ Dencho Gugutkov; George Altankov; José Carlos Rodríguez Hernández; Manuel Monleó 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 456 KB

## Abstract Adhesion of human fibroblast to a family of fibronectin (FN) coated model substrates consisting of copolymers of ethyl acrylate and hydroxyl ethylacrylate in different ratios to obtain a controlled surface density of OH groups was investigated. Cell adhesion and spreading surprisingly