Fibre-optic glucose biosensor using enzyme membranes with 2-D crystalline structure
✍ Scribed by Angela Neubauer; Dietmar Pum; Uwe B Sleytr; Ingo Klimant; Otto S Wolfbeis
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 708 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0956-5663
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✦ Synopsis
Two-dimensional crystalline bacterial surface layers (S-layers) composed of identical (glyco)protein subunits turned out to be ideal matrices for immobilizing monolayers of functional macromolecules. Due to their crystalline character, S-layers exhibit a characteristic topography with a defined arrangement and orientation of functionalities, which is a prerequisite for a reproducible and geometrically defined binding of biomolecules. The proteinic nature of S-layers provides an adequate microenvironment for immobilized biomolecules, frequently along with the effect of a high retention of biological activity and enhanced stability towards drying.
For the development of a fibre-optic glucose biosensor, monomolecular layers of glucose oxidase were covalently immobilized on the surface of Slayer ultrafiltration membranes. During glucose measurements, the enzyme monolayer was attached to the transducer, an oxygen optode containing a ruthenium(II) complex whose fluorescence is dynamically quenched by molecular oxygen. The performance of the sensor, in terms of response time, linear range and stability, is comparable to existing optodes. Given its minute size, the system presented is considered to hold great promise for the development of micro-integrated optical biosensors.