Fibre-optic biosensor based on luminescence and immobilized enzymes: Microdetermination of sorbitol, ethanol and oxaloacetate
✍ Scribed by Gautier, Sabine M. ;Blum, Loïc J. ;Coulet, Plerre R.
- Publisher
- John Wiley and Sons
- Year
- 1990
- Weight
- 465 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
We have investigated highly selective and ultrasensitive biosensors based on luminescent enzyme systems linked t o optical transducers. A fibre-optic sensor with immobilized enzymes was designed; the solid-phase bioreagent was maintained in close contact with the tip of a glass fibre bundle connected t o the photomultiplier tube of a luminometer. A bacterial luminescence fibre-optic sensor was used for the microdetermination of NADH. Various NAD(P)-dependent enzymes, sorbitol dehydrogenase, alcohol dehydrogenase and malate dehydrogenase, were co-immobilized on preactivated polyamide membranes with the bacterial system and used for the microdetermination of sorbitol, ethanol and oxaloacetate at the nanomolar level with a good precision.