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FGF2 and dexamethasone increase the production of hyaluronan in two-dimensional culture of elastic cartilage-derived cells: in vitro analyses and in vivo cartilage formation

✍ Scribed by Akiko Yokoyama; Takeshi Muneta; Akimoto Nimura; Hideyuki Koga; Tomoyuki Mochizuki; Yuiro Hata; Ichiro Sekiya

Publisher
Springer-Verlag
Year
2007
Tongue
English
Weight
498 KB
Volume
329
Category
Article
ISSN
0302-766X

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## Abstract In this study a three step culture system, 2D‐3D sequential culture __in vitro__ and further implantation __in vivo__ was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Five‐day‐old embryoid bodies were plated for chondrogenic induction for 27 days (