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Fetal and Adult Hematopoietic Stem Cells Require β1 Integrin Function for Colonizing Fetal Liver, Spleen, and Bone Marrow

✍ Scribed by Alexandre J Potocnik; Cord Brakebusch; Reinhard Fässler

Book ID
117722644
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
436 KB
Volume
12
Category
Article
ISSN
1074-7613

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Multicolor immunofluorescence and flow c
Multicolor immunofluorescence and flow cytometry utilizing cascade blue to purify murine hematopoietic stem cells from fetal liver and bone marrow
✍ Christopher J. Donahue; Christopher Fennie; Ricardo Villacorta; Hank La; Laurenc 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 289 KB 👁 1 views

Background: Here we demonstrate the utility of cascade blue (CB), to purify hematopoietic stem cells by flow cytometry. Multicolor immunofluorescence and the sensitivity (signal-to-noise) of the fluorochromes are essential for the identification and isolation of rare stem cell populations. Methods: