Fertilization of cumulus-free, zona-intact mouse ova in vitro at high and low sperm concentrations
β Scribed by Stanger, J. D. ;Quinn, P.
- Book ID
- 111662841
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1982
- Weight
- 671 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0148-7280
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## Abstract Sperm penetration of zonae and fertilization of mouse eggs in vitro was studied as a function of sperm concentration over the range 10^3^β10^6^ cells/ml. Maximal fertilization was obtained at 10^5^ sperm/ml with markedly reduced levels seen at lower concentrations. Eggs inseminated at l
## Abstract Zonaβfree eggs were introduced to fresh or preincubated sperm suspensions and the penetration of eggs by foreign spermatozoa was examined, as evidenced by enlargement of the sperm head and formation of the male pronucleus. It was found that zonaβfree hamster eggs can be penetrated by gu