Ferrocenyl glycopeptides as electrochemical probes to detect autoantibodies in multiple sclerosis patients' sera
✍ Scribed by Feliciana Real-Fernández; Amélie Colson; Jérome Bayardon; Francesca Nuti; Elisa Peroni; Rita Meunier-Prest; Francesco Lolli; Mario Chelli; Christophe Darcel; Sylvain Jugé; Anna Maria Papini
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2008
- Tongue
- English
- Weight
- 293 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
Glycopeptide analogues of CSF114(Glc), modified at N‐terminus with new ferrocenyl carboxylic acid and a new ferrocenyl‐thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these “electrochemical probes” did not affect autoantibody recognition both in SP‐ELISA and in inhibition experiments. By electrochemical monitoring the interactions of the modified peptides Fc‐CSF114(Glc) and 4‐FcPhP(S)Abu‐CSF114(Glc) with the autoantibodies, we demonstrated that autoantibodies could be detected with a sensitivity comparable to ELISA method. The new electrochemical probes can be proposed to characterize autoantibodies as biomarkers of multiple sclerosis by a simple, rapid, and reproducible cyclic voltammetry‐based diagnostic methodology. © 2008 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90: 488–495, 2008.
This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [email protected]