Ferrocene Branched Chitosan for the Construction of a Reagentless Amperometric Hydrogen Peroxide Biosensor

✍ Scribed by Ariel Garcia; Carlos Peniche-Covas; Belkis Chico; Benjamin K. Simpson; Reynaldo Villalonga

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 129 KB
• Volume: 7
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200700032

✦ Synopsis

Abstract

Chitosan was chemically branched with ferrocene moieties and further used as a support for the immobilization of horseradish peroxidase on a glassy carbon electrode. The reagentless biosensor device showed a linear amperometric response toward hydrogen peroxide concentrations between 35 × 10^−6^ M and 2.0 × 10^−3^ M. The biosensor reached 95% of the steady‐state current in about 20 s and its sensitivity was 28.4 × 10^−3^ µA · M^−1^. The enzyme electrode retained 94% of its initial activity after 2 weeks of storage at 4 °C in 50 × 10^−3^ M sodium phosphate buffer at pH 7.0.

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