Femtosecond optical transfection of cells:viability and efficiency
β Scribed by Stevenson, D.; Agate, B.; Tsampoula, X.; Fischer, P.; Brown, C. T. A.; Sibbett, W.; Riches, A.; Gunn-Moore, F.; Dholakia, K.
- Book ID
- 115406393
- Publisher
- Optical Society of America
- Year
- 2006
- Tongue
- English
- Weight
- 339 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1094-4087
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## Abstract We demonstrate the advantages of a dynamic diffractive optical element, namely a spatial light modulator (SLM) for the controlled and enhanced optoinjection and phototransfection of mammalian cells with a femtosecond light source. The SLM provides full control over the lateral and axial
## Abstract Cell transfection using femtosecond lasers is gaining importance for its proven ability to achieve selective transfection in a sterile and relatively nonβinvasive manner. However, the net efficiency of this technique is limited due to a number of factors that ultimately makes it difficu