Femoral vessels cross-clamping: The reliability of a method for sciatic nerve ischemia–reperfusion injury
✍ Scribed by Mohsen Nouri; Mohammadreza Rasouli; Reza Rahimian; Vafa Rahimi-Movaghar; Ahmad-Reza Dehpour
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 63 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0738-1085
No coin nor oath required. For personal study only.
✦ Synopsis
Regarding the article Ischemia-Reperfusion Injury of the Peripheral Nerve: An Experimental Study (Saray et al.) 1 published in your journal, we want to mention some points.
This article describes a method for inducing ischemia-reperfusion (I/R) injury in rat sciatic nerve and has been referred to in several recent articles in which this method has been used. 2,3 This article, like others suggesting a method for I/R, has used different time intervals for the reperfusion course, including no reperfusion (only ischemia), 1 h, 2 h, 3 h, 12 h, 24 h, 48 h, 7 days, 14 days, and 21 days. From the point of view that several time intervals and various assessments such as sciatic function index (SFI), levels of hydroperoxides, and pathologic evaluations have been included, this is a very unique study. However, there are some confusing points that cast doubts on the results. The authors claimed that ''Following 3 h of ischemia in group II, the SFI decreased from normal function (SFI near 0) to À24 6 3.0 at first week, 19 6 3.7 at 2 weeks, and 16 6 2.2 at 3 weeks, 1 '' where group II is the ischemia group with no reperfusion. Again, in Figure 2 of this article, it can be easily seen that all the animals, no matter which group they belong to, are observed for the entire 3 weeks. 1 It seems that the authors have confused the concept of reperfusion injury; for example, when an animal is categorized in the 1-h reperfusion group, it should be evaluated functionally 1 h after ischemia, and if we continue to follow its function