✦ LIBER ✦
Feeding of nutrients delays apoptotic death in fed-batch cultures of recombinant NSO myeloma cells
✍ Scribed by D. J. DiStefano; G. E. Mark; D. K. Robinson
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 290 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
The addition of concentrated nutrient solutions in fed-batch cultures of recombinant GS-NS0 cells delays the onset of apoptosis by 24 to 48 hours and decreases the specific rate of apoptotic death approximately two-fold. This decrease in apoptosis appears primarily responsible for the increase in culture longevity that results in five-to ten-fold increases in final product concentrations in fed-batch cultures.