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Fed-batch xylitol production with two recombinant Saccharomyces cerevisiae strains expressing XYL1 at different levels, using glucose as a cosubstrate: A comparison of production parameters and strain stability

✍ Scribed by Nina Q. Meinander; Bärbel Hahn-Hägerdal


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
244 KB
Volume
54
Category
Article
ISSN
0006-3592

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✦ Synopsis


charomyces cerevisiae strains expressing the XYL1 gene, 1997.

coding for xylose reductase (XR), at different levels, the Keywords: xylitol production; recombinant Saccharo-'low XR strain' at 0.51 U/mg and the 'high XR strain' at myces cerevisiae; protein burden; plasmid stability 10.8 U/mg, was compared in batch and fed-batch culture. Xylose was not consumed in the presence of high glucose concentrations, because both sugars are transported by the glucose transport system, which has a higher affinity