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Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces

✍ Scribed by Jordan M. Nechvatal; Jeffrey L. Ram; Marc D. Basson; Phanramphoei Namprachan; Stephanie R. Niec; Kawsar Z. Badsha; Larry H. Matherly; Adhip P.N. Majumdar; Ikuko Kato


Book ID
116750418
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
578 KB
Volume
72
Category
Article
ISSN
0167-7012

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We present a n application o f image analysis for the direct quantification o f PCR products after gel electrophoresis and ethidium bromide staining o f DNA. This procedure has been applied t o the development of a n assay based on competitive PCR for the measurement o f the degree o f amplification