An expression system for trypsin
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John R. Vasquez; Luke B. Evnin; Jeffrey N. Higaki; Charles S. Craik
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Article
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1989
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John Wiley and Sons
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English
โ 800 KB
The eukaryotic serine protease, rat anionic trypsin, and various mutants created by site-directed mutagenesis have been heterologously expressed in Escherichiu coZi. The bacterial alkaline phosphatase (phoA) promoter was used to control the expression of the enzymes in an induced or constitutive fas