Fatty Acid Anilides as Internal Standards for High Performance Liquid Chromatographic Analyses of Valeriana officinalis L. and Other Medicinal Plants
✍ Scribed by Nigel B. Perry; Elaine J. Burgess; Stephen D. Lorimer; John W. van Klink
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 572 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
Fatty acid anilides are proposed as internal standards for high performance liquid chromatographic (HPLC) analyses of medicinal plants. A one-pot synthesis of the C5 to C,, anilides is described. Reverse-phase HPLC showed that the synthesized anilides covered the optimum range of k' values for mobile phases ranging from 50 to 90% acetonitrile in water. There was an approximately linear relationship between fatty acid chain length and log k ' under a variety of isocratic conditions. For a linear gradient of mobile phase composition, there was an approximately linear relationship between fatty acid chain length and k'. The development of an extraction and HPLC analysis method for the biologically active components valtrate and valerenic acid in Valerianu oficinalis is described including use of Cll fatty acid anilide as an internal standard. The levels of these components were similar in both fresh and dried valerian.