Fate mapping the avian neural plate with quail/chick chimeras: Origin of prospective median wedge cells
✍ Scribed by Schoenwolf, Gary C. ;Bortier, Hilde ;Vakaet, Lucien
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 930 KB
- Volume
- 249
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
The origin of prospective M cells, which are median neuroepithelial cells that become wedge-shaped during bending of the neural plate and eventually form the midline floor of the neural tube, was determined by constructing quailkhick chimeras and using the quail nucleolar marker to identify quail donor cells in chick host blastoderms. Two possible sites of prospective Mcell origin in the epiblast were examined: a single, midline rudiment located just rostral to Hensen's node and paired rudiments flanking the cranial part of the primitive streak. Our results suggest that M cells arise exclusively from the midline, prenodal rudiment. From this rudiment, M cells extend caudally throughout the entire length of the neuroepithelium. This new information on the origin of prospective M cells will aid in the analysis of their role in neurulation.