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Fat metabolism in higher plants. The determination of acyl-acyl carrier protein and acyl coenzyme A in a complex lipid mixture

✍ Scribed by M. Mancha; G.B. Stokes; P.K. Stumpf

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 508 KB
Volume: 68
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90655-7

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✦ Synopsis

A method has been developed that permits the quantitative analysis of [W]acyl-acyl carrier proteins and [W] acyl CoAs from a typical reaction mixture. The method is based on (a) the initial extraction of free fatty acids and the less polar lipids into petroleum ether from aqueous isopropanol; (b) the precipitation of [Wlacyl-acyl carrier proteins in the presence of ammonium sulfate and chloroform-methanol; and (c) the final separation of acyl CoAs from the more polar lipids by selective adsorption on neutral alumina gel. All fractions can then be analyzed for the composition of complex lipids and W-labeled fatty acids by the usual methods.

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