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Fast, simple and cost-effective determination of thiopental in human plasma by a new HPLC technique

✍ Scribed by Gilberto Coppa; Roberto Testa; Anna Margarucci Gambini; Ivano Testa; Massimo Tocchini; Anna Rita Bonfigli

Book ID
117074141
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
87 KB
Volume
305
Category
Article
ISSN
0009-8981

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A simple and sensitive reversed-phase HPLC method with UV detection was developed and validated for the quantitation of 5-fluorouracil (5-FC) in human plasma. After acidification and salting out, 5-FU was extracted into ethyl acetate and back-extracted into a basic buffer. The extract was adjusted t