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Fast intracellular pH determination in single cells by flow-cytometry

✍ Scribed by G. Valet; A. Raffael; L. Moroder; E. Wünsch; G. Ruhenstroth-Bauer

Book ID
105048334
Publisher
Springer
Year
1981
Tongue
English
Weight
249 KB
Volume
68
Category
Article
ISSN
0028-1042

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## Abstract ## Background Two calibration methods have been proposed for determining the relation between the fluorescence ratio of a pH‐sensitive fluorescent indicator and intracellular pH (pH~i~). The first method uses nigericin to clamp pH~i~ to external pH (pH~e~) and the second is the null po