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Fast high-performance liquid chromatographic purification of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase

✍ Scribed by L.R. Jacob; H. Vollert; M. Rose; K.-D. Entian; L.J. Bartunik; H.D. Bartunik

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
840 KB
Volume
625
Category
Article
ISSN
1873-3778

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Purification of phosphoenolpyruvate carb
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✍ Paolo Tortora; Giorgio M. Hanozet; Andrea Guerritore πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 733 KB

Electrophoretically homogeneous phosphoenolpyruvate carboxykinase (EC 4.1.1.49) from Saccharomyces cerevisiae was obtained in high yields by means of a two-step purification procedure consisting of ion-exchange chromatography and alhnity chromatography on adenosine 5'-monophosphate-Sepharose 4B. In