Fast high-performance liquid chromatographic determination ofR(+)[(5,6-dichloro-2,3,9,9a-tetrahydro-3-oxo-9a-propyl-1-H-fluoren-7-yl)oxy]acetic acid in human plasma and urine
✍ Scribed by Hsieh, J. Y-K. ;Maglietto, B. K. ;Bayne, W. F.
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 400 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
A simple and rapid high-performance liquid chromatographic method for the determination of R(+)[(5,6-dichloro-2,3,9,9atetrahydro-3-oxo-9a-propyl-l-H-fluoren-7-yl)oxy]acetic acid (/) in human plasma and urine is described. The method utilizes Bond-El& cartridge to facilitate the drug extraction. Analysis is performed on a short reversed-phase column with a mobile phase consisting of acetonitrile and phosphate buffer and quantification is carried out by ultraviolet detection with a wavelength set at 340 nrn. The method is linear and reproducible for both plasma and urine analyses (0.25-50 pg/mL) with the detection limit of 125 ng/mL of plasma and urine. Plasma and urine concentrations of I at selected time intervals following I.V. administration of single rising doses are presented.