Fast enzymatic preparation of l-DOPA from tyrosine and molecular oxygen: a potential method for preparing [15O]l-DOPA
✍ Scribed by Jacques F. Maddaluno; Kym F. Faull
- Book ID
- 103922364
- Publisher
- Elsevier Science
- Year
- 1990
- Weight
- 554 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0883-2889
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✦ Synopsis
A fast, simple and inexpensive enzymatic preparation of 3,4-dihydroxyphenyl+alanine (L-DOPA) from molecular oxygen and tyrosine using mushroom tyrosinase is described. The theoretical incubation time for production of ["OIL-DOPA with maximal specific activity from ['50]0, can be calculated to be about 3 min. In practice, using a specially-designed glass reaction chamber to facilitate the incorporation of gaseous molecular oxygen into L-DOPA with zero lag-time, a 3 min reaction with 1% oxygen in nitrogen results in the formation of approx. 3.9 pmol of L-DOPA, representing conversion of about 14% of the tyrosine substrate. Given access to a supply of ['50]Oz, the method should be applicable to the preparation of ['50]~-DOPA for use as a PET tracer.