Fast detection of added soybean proteins in cow's, goat's, and ewe's milk by perfusion reversed-phase high-performance liquid chromatography
✍ Scribed by Esther Espeja; M. Concepción García; M. Luisa Marina
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 109 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
In order to detect possible adulterations in milk due to addition of soybean proteins a a perfusion reversed-phase chromatographic column has been used with a linear acetonitrile-water-0.1% trifluoroacetic acid binary gradient for the determination of soybean proteins in cow's, goat's, and ewe's milk spiked with such proteins. The method permitted the simultaneous separation of soybean and milk proteins in less than 2 minutes at a flow-rate of 5 mL/min and a temperature of 608C. The method also enabled the quantitative analysis of soybean proteins in spiked milk, detecting up to 30 lg of soybean proteins/mL of milk. The method was validated by evaluation of its linearity, precision, and robustness and the possible existence of matrix interferences was also investigated for some of the milks. Although the external standard calibration method enabled the quantitation of soybean proteins in most of the milks examined, there were some cases in which the standard addition method was required.