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Fast Biosynthesis of GFP Molecules: A Single-Molecule Fluorescence Study

✍ Scribed by Alexandros Katranidis; Diaa Atta; Ramona Schlesinger; Knud H. Nierhaus; Theodora Choli-Papadopoulou; Ingo Gregor; Michael Gerrits; Georg Büldt; Jörg Fitter

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
500 KB
Volume
48
Category
Article
ISSN
0044-8249

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✦ Synopsis

Abstract

It's not easy being green: Real‐time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single‐molecule‐sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).magnified image

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