Fast [18F]FDG synthesis by alkaline hydrolysis on a low polarity solid phase support
✍ Scribed by C. Lemaire; Ph. Damhaut; B. Lauricella; C. Mosdzianowski; J.-L Morelle; M. Monclus; J. Van Naemen; E. Mulleneers; J. Aerts; A. Plenevaux; C. Brihaye; A. Luxen
- Book ID
- 102372260
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- French
- Weight
- 144 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0022-2135
- DOI
- 10.1002/jlcr.572
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✦ Synopsis
Abstract
The synthesis of 2‐deoxy‐2‐[^18^F]fluoro‐D‐glucose ([^18^F]FDG) has been simplified by the use of a ^t^C18 Sep Pak cartridge to effect purification and hydrolysis of the tetraacetylated [^18^F]fluoro‐glucose compound ([^18^F]TAG). After radiolabelling, this derivative was trapped on a solid phase extraction (SPE) cartridge and the residual reaction solvent (CH~3~CN), reagents (K222, K~2~CO~3~,…) and by‐products removed by washing the support with water. After this cleaning step, the acetyl groups were cleaved on the same ^t^C~18~ column using 2N sodium hydroxide. This fast reaction proceeded near quantitatively (>98%) at room temperature in less than 2 min. The [^18^F]FDG was then recovered with a small amount of water, neutralized with a slight excess of 2N hydrochloric acid, buffered for pH with a citrate solution and finally purified on a neutral alumina oxide and a second ^t^C18 column. After filtration, the radiochemical yield of this [^18^F]FDG isotonic solution after more than 100 production runs was found to be very reliable and reproducible (70±6% decay corrected). The synthesis time was about 22 min. Quality controls showed that the radiochemical purity was higher than 98% and in any case no [^18^F]FDM was detected. Only traces of 2‐chloro‐2‐deoxy‐glucose (ClDG) were found in the final sample (64±9 μg/ batch of 16 ml). [^18^F]FDG specific activity averaged between 1 and 20 Ci/µmol (EOS). No evaporation and use of ion retardation resin (AG11A8) are required. Moreover, this new approach is suitable for complete remote operation using available single use medical components. Copyright © 2002 John Wiley & Sons, Ltd.