Farm-grown mushrooms (Agaricus campestris) in the diet of rats do not affect plasma and liver cholesterol concentrations
✍ Scribed by Beynen, A. C. ;Fielmich, A. M. ;Lemmens, A. G. ;Terpstra, A. H. M.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 272 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0027-769X
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✦ Synopsis
Wissenschaftliche Kurzberichte/Short Communications
The FTIR spectra of soybean saponins Ab and Bb exhibited bands representative of the group frequencies associated with the compounds, as shown in Fig. 2 and3, respectively. Bands at 1753.2 and 1233.0cm-' in the spectrum of saponin Ab were ascribed to the acetoxyl groups from a-L-arabinopyranosyl of the sugar chain linked at the C-22 site of soyasapogenol A. A strong absorption at 1762.2 cm-' was interpreted as a vibrational band of the carboxyl group from the first sugar chain linked at C-3 of soyasapogenol B. In the case of saponin Ab, however, this band was obscured by an IR absorption signal at 1756.2cm-' from acetyl groups. Bending of C-H bonds (in-plane) from acetyl moieties of saponin Ab was noted at 1380.9cm-'. For saponin Bb, stretching of CH3 groups of the sugar chain gave rise to signal at 2946.1 cm-'. This was also the case for CH3 moieties of acetyl groups of saponin Ab. In triterpen, IR absorption of hydroxyl groups gave rise to characteristic bands at 3405.1 cm-' and 3414.8 cm-' for saponins Bb and Ab, respectively. Strong absorption signals noted at 1049.2 cm-' for saponin Bb and 1041.5 and 1076.2 cm-' for saponin Ab were assigned to C-0 stretching. The C=C stretching was observed at 1639.4cm-' for saponin Ab and at 1637.5 cm-' for saponin Bb.
The FI'IR spectra presented in this paper are similar to spectra of individual saponins of group A and B measured by continous wave infrared spectroscopy [6-81. In quoted articles IR spectra of Ab saponin showed the peaks at 174Ocm-' and 1240cm-' assigned to aliphatic acetoxyl group. The IR spectra of Bb saponin did not show the two absorptions, what indicated that this saponin was not acetylated in soybean seed.
In conclusion we want to emphasize that in relation to classical infrared spectroscopy FITR records spectra of saponins faster and peaks are sharper. This statement refers especially to the region 1000-500 cm-'.