Failure to trigger the autolytic enzymes in minicells of Escherichia coli
✍ Scribed by Zdzislaw Markiewicz; Joachim-Volker Höltje
- Book ID
- 109317537
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 273 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0378-1097
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In vitro synthesis of enzymes of the tryptophan (trp) operon of E. coli was studied in an extract prepared from E. coli, which is programmed with purified DNA from trp transducing phages with mutations that effect the expression of the trp genes in various ways. Our results show that control of tran
A protein fraction, called At (= anti termination) factor, has been isolated from extracts of E. coli and partially purified. The At factor stimulates the synthesis in vitro of anthranilate synthetase, an enzyme encoded by two genes of the tryptophan (trp) operon, but has no effect on the synthesis