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Failure of Ca2+-activated, CREB-dependent transcription in astrocytes

✍ Scribed by Peter D. Murray; Tami J. Kingsbury; Bruce K. Krueger

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
252 KB
Volume
57
Category
Article
ISSN
0894-1491

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✦ Synopsis

Abstract

Astrocytes participate in signaling via Ca^2+^ transients that spread from cell to cell across a multicellular syncytium. The effect, if any, of these Ca^2+^ waves on the transcription of Ca^2+^/cAMP‐regulatory element binding protein (CREB)‐dependent genes is not known. We report here that, unlike neurons, increasing intracellular Ca^2+^ in cultured mouse cortical astrocytes failed to activate CREB‐dependent transcription, even though CREB was phosphorylated at serine 133. In contrast, both CREB phosphorylation and CREB‐dependent transcription were robustly stimulated by increasing cAMP. The failure of Ca^2+^‐activated transcription in astrocytes was correlated with the absence of CaMKIV, a Ca^2+^‐dependent protein kinase required for Ca^2+^‐stimulated gene transcription in neurons. The inability of Ca^2+^ to signal via CaMKIV may insulate CREB‐dependent gene transcription in astrocytes from activation by Ca^2+^ waves. © 2008 Wiley‐Liss, Inc.

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