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Failure of a selective medium to isolate Haemophilus somnus strains

✍ Scribed by JM KWIECIEN; PB LITTLE

Book ID
114888802
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
397 KB
Volume
66
Category
Article
ISSN
0005-0423

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An E. coli strain deleted in the region malasd is used for the selection of conditional or auxotrophic mutants. Thermosensitive and auxotrophic strains have thus been isolated on plates. After selection in liquid medium, a strain has been isolated which is sensitive to excess one-carbon metabolites.