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FAD assembly and thylakoid membrane binding of ferredoxin:NADP+ oxidoreductase in chloroplasts

✍ Scribed by Yayoi Onda; Toshiharu Hase

Book ID
117106447
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
230 KB
Volume
564
Category
Article
ISSN
0014-5793

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Purification of membrane-bound ferredoxi
Purification of membrane-bound ferredoxin: NADP+oxidoreductase and of plastocyanin from a detergent extract of washed thylakoids
✍ Hans C. P. Matthijs; Deborah Moore; Sean J. Coughlan; Geoffrey Hind πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 508 KB

A method is described for the isolation and purification of ferredoxin-NADP + oxidoreductase (FNR, E.C. 1.18.1.2) and plastocyanin from spinach thylakoids. FNR is recovered from pools which are loosely and tightly bound to the membrane, with minimal disruption of pigment-protein complexes; yields ca