## Abstract Peripheral lymphocytes from 3 scleroderma patients with systemic disease and from 3 normal controls were examined for frequencies of chromosomal aberration and sister chromatid exchange (SCE). Significant increases in the levels of chromosomal anomaly and SCE were found for all 3 patien
Factors underlying variation in spontaneous and clastogen-induced sister chromatid exchanges and chromosome breakage frequencies
โ Scribed by S. S. Wasserman; M. M. Cohen; S. Schwartz; B. H. Margolin
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 443 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0893-6692
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Human leukocyte cultures were pulse-treated with the trifunctional alkylating mutagen trenimon in a final concentration of 10(-7) M for 15--20 h after culture start, i.e., in the G1 phase of the cell cycle. At 24 h after culture start bromodeoxyuridine (BUdR) was added to the trenimon-treated cultur
The incidence of structural chromosome aberrations and the rate of sister chromatid exchanges (SCE) was investigated in lymphocyte cultures from a patient with typical Fanconi's anemia and his parents. The rate of SCEs was found to be normal. In experiments with the alkylating agent Trenimon the SCE