✦ LIBER ✦

Factors that affect the extraction of xyloglucan from the primary cell walls of suspension-cultured rose cells

✍ Scribed by Hans G. Edelmann; Stephen C. Fry

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 630 KB
Volume: 228
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(92)84135-f

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✦ Synopsis

The extraction of xyloglucan from Rosa suspension-culture cell walls (not depectinated in hot aqueous solutions) with alkali was dependent on the temperature, with 2-3 times more being extracted at 37" than at 25". At 25', 6wNaOH was the optimal concentration, but it extracted only 2S-30% ofthexyloglucan within 24 h; efficient extraction took 2-3 weeks. After pre-treatment of cell walls with 6~ NaOH at 25", water extracted more xyloglucan in 24 h than did additional 6~ NaOH.

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