Factors influencing shoot regeneration from cotyledonary explants ofCucumis melo

✍ Scribed by Randall P. Niedz; Sandra Schiller Smith; Kerry B. Dunbar; Christine T. Stephens; Harry H. Murakishi

• Book ID: 104620599
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 348 KB
• Volume: 18
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00043400

✦ Synopsis

Cotyledonary explants of 4-day-old Cucumis melo cv. 'Hale's Best Jumbo' in vitro seedlings showed maximum initiation of shoot buds when cultured onto a revised Murashige & Skoog medium supplemented with 5/~M indole-3-acetic acid and 5/~M benzylaminopurine and cultured at 25-29°C under low light intensity (5-30#molm-2s-~). Subculture of the shoot buds onto the same medium without auxin and supplemented with 3 #M benzylaminopurine caused the development of shoots from 30% of the buds. The presence of abscisic acid significantly increased the number of explants producing shoot buds. Bud initiation was affected by genotype, seedling age, light intensity, and temperature. Addition of gibberellic acid, thidiazuron or silver nitrate to regeneration medium did not improve either bud initiation or shoot regeneration.