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Factors Controlling Phase Separation in Water−Salt Solutions of DNA and Polycations

✍ Scribed by Izumrudov, V. A.; Wahlund, P.-O.; Gustavsson, P.-E.; Larsson, P.-O.; Galaev, I. Yu.


Book ID
127267130
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
92 KB
Volume
19
Category
Article
ISSN
0743-7463

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Phase Separations in Water–Salt Solution
✍ Per-Olof Wahlund; Vladimir A. Izumrudov; Per-Erik Gustavsson; Per-Olof Larsson; 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB

## Abstract Formation of insoluble polyelectrolyte complexes (PECs) between RNA and polycations was followed by measuring the residual RNA absorbance in the solution after separation of the precipitate. The polycations studied were poly(__N__,__N′__‐dimethyldiallylammonium) chloride (pendant type)

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Destruction of interpolyelectrolyte complexes (PEC) formed by DNA and different poly(Nalkyl-4-vinylpyridinium) cations was achieved by addition of low molecular weight electrolytes. Monitoring of PEC dissociation was carried out by fluorescence quenching using the ability of the cationic dye ethidiu