Factors affecting the ultramicro-determination of tryptophan, kynurenine and their derivatives

✍ Scribed by Anwar A. Hakim

• Publisher: Elsevier Science
• Year: 1959
• Tongue: English
• Weight: 560 KB
• Volume: 21
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(59)80159-8

✦ Synopsis

SUMIMARY
The ficturs iffecting the ultramicrodetermination of tryptophian and kynurenine were investisated by paper electrophoresis, paper chromalogriphy and spectrophotometry. The electrolyte used in electrophoresis influences the mobility, the separation and the ultraviolet spectrit of tryptophan and kynurenine. The pre-treatment of filter paper for chromatogriphy did not influence the (h_{p}) of tryptophan and kynurenine, hut interfered with their quantitativedeterminition. The effect of ultraviolet irradiation of (d-, l-), and (d l)-tryptophan as well ats (d-, l-), and (d l-k y-n u r e n i n e) wats studied.