Factors affecting the performance of the BHK 21 cell transformation test
β Scribed by M.R. Daniel; J.M. Dehnel
- Book ID
- 113250129
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 68 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0165-1161
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## Abstract Although BHK 21 cells remain refractory to direct transformation by SV40 virus even at multiplicities of infection as high as 10^4^ PFU per cell, transformed derivatives may be produced by cocultivation of the untransformed cells with monkey cells infected with SV40 virus. The characte
B H K 21/13 cells, 50 gcnerutioris f r o m cloning, are rransplaiitablc~ owing to the prcwiice qf a sniall proportion (about 0.00i 74) of' highly tuniorigenic variarir cells. Noti-tuiriorigeriic cells can easily be i.solated,froni thcsr sfoclis by srcloning. With passagc in vitro the proportion of