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Factors affecting the kinetics of light emission from crude and purified firefly luciferase

โœ Scribed by Marlene DeLuca; Jon Wannlund; W.D. McElroy

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
369 KB
Volume
95
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Crude and purified firefly luciferase have been used to assay ATP from 0.2 pmol to 2 pmol. Over this range of ATP concentrations, there is a large change in the kinetics of light emission. At the lowest concentrations of ATP, light emission rises to a maximum and remains constant for a minute or longer. As the concentration of ATP is increased, the peak light intensity increases and the decay rate of light increases significantly. This is true for both the crude as well as the purified enzyme. High concentration of sodium arsenate as well as other salts inhibit the peak light emission and prevent the decay in light intensity which is due to nroduct inhibition. It is possible to obtain almost any type of kinetics by manipulating the experimental conditions.

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