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Factors affecting the enumeration of coliphages in sewage and sewage-polluted waters

✍ Scribed by A. H. Havelaar; W. M. Hogeboom

Publisher: Springer Netherlands
Year: 1983
Tongue: English
Weight: 577 KB
Volume: 49
Category: Article
ISSN: 0003-6072
DOI: 10.1007/bf00399318

No coin nor oath required. For personal study only.

✦ Synopsis

The count of coliphages in naturally polluted waters was found to be dependent on many experimental factors. If Escherichia coli C was used as a host strain, consistently higher counts were obtained than with other strains (B,K-12-derivatives). This could be explained partly by the absence of a restriction system in C. A nutrient medium (modified Scholtens' agar, MSA) was developed with optimal concentrations of calcium- and magnesium-ions. MSA was compared with other media used for phage work and was found to give higher counts than all but one medium, Phage Assay Agar (PAA), which performed equally. If plating was done in a single agar layer in a large-size Petri-dish, higher counts were found than with the well-known double-agar-layer method.

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